Plasmid

Part:BBa_I735006:Design

Designed by: Giovanni Russo   Group: iGEM07_Naples   (2007-09-28)


Cloning CDS PHO80 mutated in EX-Ptet-S-rbsRFP-P


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 78
    Illegal PstI site found at 961
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 78
    Illegal PstI site found at 961
    Illegal NotI site found at 7
    Illegal NotI site found at 954
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 78
    Illegal PstI site found at 961
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 78
    Illegal PstI site found at 961
    Illegal AgeI site found at 657
    Illegal AgeI site found at 769
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2042


Design Notes

PHO80CDS with RFP is our output


Source

PHO80CDS was amplified from DNA yeast

References